Time-resolved proteomics of adenovirus infected cells

Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells□S

Adenoviruses replicate primarily in the host cell nucleus, and it is well established that adenovirus infection affects the structure and function of host cell nucleoli in addition to coding for a number of nucleolar targeted viral proteins. Here we used unbiased proteomics methods, including high throughput mass spectrometry coupled with stable isotope labeling by amino acids in cell culture (...

Electron microscopy of cells infected with adenovirus type 2.

An electron microscope study of two strains of human adenovirus type 2 revealed the production of intranuclear paracrystalline formations by one of the strains. The crystals were composed of cylindrical tubules 25.0 nm in diameter arranged in a crystalline lattice with a periodicity of 75.0 nm. They appeared at 36 hr postinfection in nuclei which contained viral particles. Prolonged treatment w...

Initiation of transcription in nuclei isolated from adenovirus infected cells

Initiation of adenovirus transcription was analyzed by incubation of isolated nuclei from virus infected cells in the presence of beta-32P GTP or beta-32P ATP. Nucleotide analysis of RNA from nuclei incubated with beta-32P GTP shows that the label is incorporated exclusively into pppGp and ppGp. Under similar incubation conditions, the label from beta-32P ATP was incorporated primarily into the...

Reduplication of Nuclear Membranes in HeLa Cells Infected with Adenovirus

Previous electron microscopic studies of tissue cultures infected by type 3 adenovirus have revealed clusters of parallel membranes (1), as well as intranuclear tubules composed of lamellae (2). This communication reports the appearance of multiple membranes, probably nuclear in origin, in association with type 10 adenovirus. Adenovirus, type 10,1 was grown in HeLa cells maintained on a medium ...

The acid-soluble components of adenovirus and of adenovirus-infected cells.